Surface Minimal Bactericidal Concentration: A comparative study of active glasses functionalized with different-sized silver nanoparticles

In this work the quantification of antimicrobial properties differently sized AgNPs immobilized on a surface was studied. Three different sizes spheroidal with diameter (6, 30 and 52) nm were synthetized characterized UV–vis, SEM, TEM ICP-MS. The MIC (Minimal Inhibitory Concentration) MBC Bactericidal against Escherichia coli investigated. Then, antibacterial efficacy (R) amino-silanized glasses coated amounts three quantified by international standard ISO 22196 adapted protocol E. coli, clarifying relationship between size surface. total amount silver present an R ? 6 for each ICP-MS considered Surface (SMBC), which found to be (0.023, 0.026 0.034) ?g/cm2 AgNPs, respectively. Thus, study demonstrates that active surfaces bactericidal effect at least ? 99.9999 % could obtained using almost 100 times lower than colloidal AgNPs. immobilization reduces aggregation phenomena normally occuring in liquid media, maximizing exposed specific superficial area their direct contact bacterial cells. Starting from glass model system, our broaden way development wide range materials very low can safely applied biomedical food packaging fields.